Undifferentiated HepaRG cells - HPR101
The HepaRG™ cell line
HPR101 is a frozen vial of undifferentiated HepaRG™ cells at passage P12.
HPR101 HepaRG™ cells can be grown in-house up to passage 20 to increase their population or can be differentiated into fully functional hepatocytes-like cells.
HepaRG™ is patented. HPR101 is only available under a MTA for academic scientists or through a sub-license established between Biopredic International and researchers from commercial organisations.
Read more on how to get HPR101 undifferentiated HepaRG™ cryopreserved cells.
Download HPR101 Product Specifications
Key advantages
- Flexibility - the cells can be used at your convenience
- Reproducible experiments - identical differentiation process for each passage
- Long living - cultures remain viable and usable for 7-8 passages
- Long-term studies - well adapted to stable gene transfer
Storage and delivery conditions for HPR101 cells
Undifferentiated HepaRG™ cryopreserved cells are delivered in dry ice and can be stored for 5 years in liquid nitrogen.
Please follow the Instruction manual for the culture of the HepaRG™ cell line. The instruction manual is provided once a MTA or a sub-license agreement has been signed.
HPR101 cells, media and additives
- HPR101 - Undifferentiated HepaRG™ cells cryopreserved
0.5 mL vial with at least 1 x 106 viable cells
MEDIA FOR CELL CULTURE
- MIL700C - Basal hepatic cell medium 500 ml
- ADD710C - HepaRG™ Growth Medium Supplement with antibiotics
65.5 mL. To be combined with 500 ml of base medium - ADD711C - HepaRG™ Growth Medium Supplement
59.3 mL. To be combined with 500ml of base medium - ADD720C - HepaRG™ Differentiation Medium Supplement with antibiotics
76.7 mL. To be combined with 500 ml of base medium - ADD721C - HepaRG™ Differentiation Medium Supplement
70.1 mL. To be combined with 500 ml of base medium
HPR101 - Quick links
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Microscopic observation (x100) at passage 14 after 2 weeks in the HepaRG™ Growth Medium using ADD710, and 2 weeks in HepaRG™ Differentiation Medium using ADD720. 50 % of the cells are typical hepatocyte-like cells, which are organized in well-delineated clusters with bright canaliculi-like structures. |
Protocol for the culture of the HepaRG™ cell line
Every 2 weeks, confluent HepaRG™ progenitor monolayers are detached incubating with 0.05% trypsin solution for 5 min. Cells are collected in the HepaRG™ growth medium using ADD710 or ADD711.
After cell counting, cells are seeded in the same medium and placed in a CO2 incubator at 37° C. Medium is renewed every 2-3 days thereafter. Progenitors rapidly grow up in the first 5-6 days and are highly confluent after 2 weeks.
At this stage, HepaRG™ cells can be trypsinised and passaged in order to maintain the cell line, or incubated in the HepaRG™ differentiation medium using ADD720 or ADD721, which causes the cells to undergo a complete hepatocyte differentiation programme.
Within 2 weeks, two cell types are easily recognized: hepatocyte colonies and primitive biliary cells.
Cells can then be maintained in differentiation medium for 4 further weeks.
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