HPR101 is a frozen vial of undifferentiated HepaRG™ cells at passage P12.
HPR101 HepaRG™ cells can be grown in-house up to passage 20 to increase their population or can be differentiated into fully functional hepatocytes-like cells.
Undifferentiated HepaRG™ cryopreserved cells are delivered in dry ice and can be stored for 5 years in liquid nitrogen.
Please follow the Instruction manual for the culture of the HepaRG™ cell line. The instruction manual is provided once a MTA or a sub-license agreement has been signed.
MEDIA FOR CELL CULTURE
Microscopic observation (x100) at passage 14 after 2 weeks in the HepaRG™ Growth Medium using ADD710, and 2 weeks in HepaRG™ Differentiation Medium using ADD720.
50 % of the cells are typical hepatocyte-like cells, which are organized in well-delineated clusters with bright canaliculi-like structures.
Every 2 weeks, confluent HepaRG™ progenitor monolayers are detached incubating with 0.05% trypsin solution for 5 min. Cells are collected in the HepaRG™ growth medium using ADD710 or ADD711.
After cell counting, cells are seeded in the same medium and placed in a CO2 incubator at 37° C. Medium is renewed every 2-3 days thereafter. Progenitors rapidly grow up in the first 5-6 days and are highly confluent after 2 weeks.
At this stage, HepaRG™ cells can be trypsinised and passaged in order to maintain the cell line, or incubated in the HepaRG™ differentiation medium using ADD720 or ADD721, which causes the cells to undergo a complete hepatocyte differentiation programme.
Within 2 weeks, two cell types are easily recognized: hepatocyte colonies and primitive biliary cells.
Cells can then be maintained in differentiation medium for 4 further weeks.