HPR116 is available through a Limited Use License Agreement.
After thawing, the differentiated HepaRG™ cells can be used immediately in suspension for clearance, metabolism or transporter studies.
They can also be seeded and used as little as 4 hours later for metabolism studies in monolayer.
A few days after seeding and culture, the cells will form a co-culture of hepatocytes and of biliary-like epithelial cells. They can then be used for induction, toxicity or metabolism studies.
Read more about the main applications of HepaRG™ >>
The cells are provided in a 1 ml vial with 8 x 106 viable cells.
Cell seeding:
- One vial will seed one 96-well plate (72 000 cells/well)
- One vial will seed 16 wells of one 24-well plate (480 000 cells/well)
Please carefully respect recommended cell seeding density.
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1 mL / 8 million cells / 10% DMSO |
Thawing process |
|
1. | Thaw and dilute cells with 9 mL of thawing medium (10 ml / 8 million cells) |
2. | Centrifuge, wash and re-suspend cells with 5 mL thawing / plating medium |
3. | Count cell viability, concentration and yield |
4. | Adjust cell concentration to 0.96 million cells/mL and plate cells onto 24 MW-plate (0.5 mL / well) |
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Differentiated HepaRG™ cryopreserved cells are delivered in dry ice or in a nitrogen vapour dewar. If you receive them in dry ice, ensure shipment time did not exceed 48 hours.
HPR116 cells can be stored for 5 years in liquid nitrogen.
Morphology of the cells after thawing in 96-well plates.
From day 1 to day 2 in 670 Thawing/ Plating/ General Purpose HepaRG™ medium.
From day 2 to day 8: in 620 Maintenance/ Metabolism HepaRG™ medium.
![]() Day 0 / 4h after plating |
![]() Day 1 |
![]() Day 3 |
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The following plates are provided by Biopredic International with HPR116 cells, with a proprietary coating process to ensure proper seeding and culture of the HPR116:
Media |
Catalogue reference |
Renewal of the medium | Use |
HepaRG™ Thawing/Plating/General Purpose Medium |
MIL600C |
Every 2-3 days | - Thawing - Seeding - For applications requiring maintaining of basal hepatocyte-specific functions |
HepaRG™ Maintenance/Metabolism Medium |
MIL600C + ADD620C |
Every 2-3 days | - Maintenance and use of the cells until the limit date of use (3 weeks after seeding). - Maintains hepatocyte specific functions to higher levels. |
HepaRG™ Induction Medium |
MIL600C + ADD640C |
Daily following use of 670 | - Use for induction studies, up to 3 days |
HepaRG™ Serum-free Induction Medium |
MIL600C + ADD650C |
Daily following use of 670 | - Use for induction studies, up to 3 days |
BIOPREDIC International | customersinquiries@wepredic.com | Phone: +33 (0)2 99 14 36 14 | Parc d'Affaires de la Bretèche 35760 Saint Grégoire FRANCE