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The most innovative and useful hepatic cell line

♦ A unique and well established hepatic cell system able to produce early hepatic progenitor cells as well as completely mature human hepatocytes.

♦ The one-stop hepatic in-vitro model for:

- In-vitro ADME applications:
Inhibition assays
Drug metabolism and clearance
Metabolite ID

- In-vitro toxicity testing applications:
Hepatotoxicity (reactive metabolites, lipidosis, choleastasis etc)
Virology (HBV, HCV…)
Infection with hepatitis and other viruses for replication

- Liver assist devices/ BioArtificial Livers 
- Transgenic liver humanized animals with HepaRG™ cells
…. an ever-expanding range of applications!

♦ More economical, convenient, and predictable than fresh or cryopreserved primary human hepatocytes

♦ Available in proliferative state to be expanded and differentiated in-house, or as cryopreserved, fully differentiated and ready-to-use hepatic cells

Features of the HepaRG™ cells:
♦ Full array of functions, responses, and regulatory pathways of primary human hepatocytes including:
- Phase I and II, and transporter activities consistent with those found within a population of primary human hepatocytes
- Intact response elements, PXR, CAR and PPARa
♦ Form bile canaculi
♦ Has the potential to express major properties of stem cells
 High plasticity & complete transdifferentiation capacity

HepaRG™ model system for cholestasis prediction

Time lapse (Total recording time: 2 hours).
Chlormoprazine superior to 40µM constricts the bile lumen and provokes cholestasis.
Constriction of canaliculus lumen has been observed in presence of CPZ after 2 hours.



HepaRG support the next meeting of "Réseau MeetOchondries" who will take place on May 14-17,... Read more

SOT - March 2017

BIOPREDIC INTERNATIONAL will be present at the 56th SOT meeting on booth 1724. ... Read more

Latest publications

  • Fluorometric evaluation of CYP3A4 expression using improved transgenic HepaRG cells carrying a dual-colour reporter for CYP3A4 and CYP3A7Takafumi Ueyama, Saori Tsuji Takemi Sugiyama, Masako Tada
    Scientific Reports, Jun 2017
  • MicroRNA Hsa-miR-370-3p Suppresses the Expression and Induction of CYP2D6 by Facilitating mRNA Degradation.Zeng L, Chen Y, Wang Y, Yu LR, Knox B, Chen J, Shi T, Chen S, Ren Z, Guo L, Wu Y, Liu D, Huang K, Tong W, Yu D, Ning B
    Biochemical Pharmacology, Jun 2017
All publications on HepaRG

The original cells were isolated from a donor with cholangiocarcinoma, and the novelty of the work performed by INSERM and Biopredic International scientists was to recognize their uniqueness and determine optimum culture conditions to support either growth or function as differentiated, adult hepatic cells.
Read more about HepaRG origins.

Thus, we have not interfered with their genome but utilized it advantageously, which may account for the cells’ karyotypic stability over a number of passages. The use of proliferating cells can provide a unique opportunity to analyze signaling pathways and elucidate control mechanisms in the HepaRG.

Our scientists’ excitement grew as they realized that the HepaRG cells carried properties of early hepatic progenitor cells as well as mature hepatocytes, each condition allowing for uses of the cells that are unmatched by any other hepatic cell model. For example, HepaRG in the growth state tolerate infection by certain viruses which rarely enter differentiated, adult hepatocytes.

The predictability with which the cells can be differentiated, means that we can reliably provide cells with consistent characteristics at lower cost than primary human hepatocytes.

Scientists can utilize HepaRG in two forms: cryopreserved, differentiated cells that are convenient to both use and store; and growth-stage cells that can be grown in-house to build stock populations and then frozen or differentiated.

Biopredic International has complete user instructions to ensure maximum quality of the differentiated cells, which require non-expensive materials for their use. In fact, HepaRG™ cells actually reduce overall costs compared with cryopreserved primary human hepatocytes.

When using growth-stage cells, a common question is whether their stability and useful life are retained when they are differentiated, and with HepaRG, the answers are positive.

They can be grown and split passages eight times when used according to our recommended protocols and medium supplements (the base medium is off-the-shelf William's E medium). The differentiation process is the same across passages and cells are usable for at least four weeks once differentiation is achieved.

Since the HepaRG have not undergone transfection or genome modification, they retain a range of hepatic cell responses, meaning that they have a very broad application versatility. For instance, they have shown the propensity to develop cholestasis or steatosis when exposed to appropriate agents. Likewise, they have been used successfully in studies investigating sugar and lipid metabolism, in-vivo engraftment in rodents, 3D systems, bioartificial livers, chronic toxicity, etc.

Most importantly, Biopredic International maintains a large supply of HepaRG™ progenitor cells to serve as seed stock. This is sufficient to meet the needs of the next decade, even if growth in demand is triple our projections.

HepaRG cells deliver unique, practical and economical qualities which are unmatched today. Although new technology may emerge, they lack the in-denth knowledge of these well-known cells wich is presented in 290+ papers and posters in the last years.

BIOPREDIC International   | | Phone: +33 (0)2 99 14 36 14 | Parc d'Affaires de la Bretèche 35760 Saint Grégoire FRANCE

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